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KMID : 0861020170320030097
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Korea Journal of Herbology
2017 Volume.32 No. 3 p.97 ~ p.103
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Anti-Inflammatory Effect of Anemarrhenae Rhizoma 80% Ethanol Extract in RAW 264.7 cells
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Lee Young-Keun
Kim Cheong-Taek
Choi Hak-Joo
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Abstract
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Objective : According to recent studies, Anemarrhenae Rhizoma has anti-inflammatory activities of DW extract, but it hasn`t not yet conducted to evaluate inflammatory factors about 80% ethanol extract. Therefore, The aim of this study is to investigate the various effects of individual or combined 80% ethanol extract of Anemarrhenae Rhizoma on cell viability and various anti-inflammatory factors.
Methods : Anemarrhenae Rhizoma extract was prepared with 80% ethanol. MTT assay, ELISA, and Luminex were performed in LPS-activated RAW 264.7 cell line to measure cytotoxicity, Nitric oxide (NO), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), and cytokines (IL-1β, IL-6, and TNF-α), respectively.
Results : At concentration of 200 ㎍/㎖ Anemarrhenae Rhizoma extract, cytotoxicity was observed in RAW 264.7 cells. However, at concentration less than 100 ㎍/㎖ of Anemarrhenae Rhizoma, cytotoxicity was not observed in RAW 264.7 cells. All concentration of Anemarrhenae Rhizoma extract showed no difference of NO, and IL-1β level in RAW 264.7 cells compared with control group. In contrast, at concentration of 100 ㎍/㎖ Anemarrhenae Rhizoma extract significantly inhibited LPS-induced production of COX-2, PGE2, and LTB4 level in RAW 264.7 cells. In addition, the production of proinflammatory cytokines (IL-6, TNF-α) in LPS-induced RAW 264.7 cells was significantly decreased at concentration of all or 10, and 100 ㎍/㎖, respectively.
Conclusion : These findings demonstrate that Anemarrhenae Rhizoma has inhibitory effect on inflammatory mediators in LPS-activated RAW 264.7 cells showing possible developed as a raw material for new therapeutics to ease the symptoms related with inflammatory.
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KEYWORD
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Anemarrhenae Rhizoma, anti-inflammatory, inflammatory factors, LPS, macrophage
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